Trucks are parked at a road as authorities closed the Torkham border for NATO supply trucks at Pakistani border town of Torkham on Saturday, Nov 26, 2011. Pakistan on Saturday accused NATO helicopters of firing on two army checkpoints in the northwest and killing 25 soldiers, then retaliated by closing a key border crossing used by the coalition to supply its troops in neighboring Afghanistan. (AP Photo/Qazi Rauf)
Trucks are parked at a road as authorities closed the Torkham border for NATO supply trucks at Pakistani border town of Torkham on Saturday, Nov 26, 2011. Pakistan on Saturday accused NATO helicopters of firing on two army checkpoints in the northwest and killing 25 soldiers, then retaliated by closing a key border crossing used by the coalition to supply its troops in neighboring Afghanistan. (AP Photo/Qazi Rauf)
Soldiers of the Pakistani militia force stand guard at the Pakistani border post of Torkham as it is closed for NATO trucks carrying supplies to neighboring Afghanistan, on Saturday, Nov 26, 2011. Pakistan on Saturday accused NATO helicopters of firing on two army checkpoints in the northwest and killing 25 soldiers, then retaliated by closing a key border crossing used by the coalition to supply its troops in neighboring Afghanistan. (AP Photo/Qazi Rauf)
Pakistani security personnel stop trucks carrying supplies for NATO forces in neighboring Afghanistan at Takhtabeg check post in Pakistani tribal area of Khyber, Pakistan, on their way to Torkham border post on Saturday, Nov 26, 2011. Pakistan on Saturday accused NATO helicopters of firing on two army checkpoints in the northwest and killing 25 soldiers, then retaliated by closing a key border crossing used by the coalition to supply its troops in neighboring Afghanistan. (AP Photo/Muhammad Sajjad)
Pakistani security personnel stop trucks carrying supplies for NATO forces in neighboring Afghanistan at Takhtabeg check post in Pakistani tribal area of Khyber, Pakistan, on their way to Torkham border post on Saturday, Nov. 26, 2011. Pakistan, on Saturday, accused NATO helicopters of firing on two army checkpoints in the northwest and killing 25 soldiers, then retaliated by closing a key border crossing used by the coalition to supply its troops in neighboring Afghanistan. (AP Photo/Muhammad Sajjad)
Trucks carry supplies for NATO forces in neighboring Afghanistan are halt at Takhtabeg check post in Pakistani tribal area of Khyber, Pakistan, on their way to Torkham border post on Saturday, Nov 26, 2011. Pakistan on Saturday accused NATO helicopters of firing on two army checkpoints in the northwest and killing 25 soldiers, then retaliated by closing a key border crossing used by the coalition to supply its troops in neighboring Afghanistan. (AP Photo/Qazi Rauf)
ISLAMABAD (AP) ? Pakistan has blocked vital supply routes for U.S.-led troops in Afghanistan and demanded Washington vacate a base used by American drones after coalition aircraft allegedly killed 24 Pakistani troops at two posts along a mountainous frontier that serves as a safe haven for militants.
The incident Saturday was a major blow to American efforts to rebuild an already tattered alliance vital to winding down the 10-year-old Afghan war. Islamabad called the bloodshed in one of its tribal areas a "grave infringement" of the country's sovereignty, and it could make it even more difficult for the U.S. to enlist Pakistan's help in pushing Afghan insurgents to engage in peace talks.
A NATO spokesman said it was likely that coalition airstrikes caused Pakistani casualties, but an investigation was being conducted to determine the details. If confirmed, it would be the deadliest friendly fire incident by NATO against Pakistani troops since the Afghan war began a decade ago.
A prolonged closure of Pakistan's two Afghan border crossings to NATO supplies could cause serious problems for the coalition. The U.S., which is the largest member of the NATO force in Afghanistan, ships more than 30 percent of its non-lethal supplies through Pakistan. The coalition has alternative routes through Central Asia into northern Afghanistan, but they are costlier and less efficient.
Pakistan temporarily closed one of its Afghan crossings to NATO supplies last year after U.S. helicopters accidentally killed two Pakistani soldiers. Suspected militants took advantage of the impasse to launch attacks against stranded or rerouted trucks carrying NATO supplies. The government reopened the border after about 10 days when the U.S. apologized. NATO said at the time the relatively short closure did not significantly affect its ability to keep its troops supplied.
But the reported casualties are much greater this time, and the relationship between Pakistan and the U.S. has severely deteriorated over the last year, especially following the covert American raid that killed Osama bin Laden in a Pakistani garrison town in May. Islamabad was outraged that it wasn't told about the operation beforehand.
The government announced it closed its border crossings to NATO in a statement issued after an emergency meeting of the Cabinet's defense committee chaired by Pakistani Prime Minister Yousuf Raza Gilani.
It also said that within 15 days the U.S. must vacate Shamsi Air Base, which is located in southwestern Baluchistan province. The U.S. uses the base to service drones that target al-Qaida and Taliban militants in Pakistan's tribal region when they cannot return to their bases inside Afghanistan because of weather conditions or mechanical difficulty, said U.S. and Pakistani officials, speaking on condition of anonymity to discuss sensitive strategic matters.
The government also plans to review all diplomatic, military and intelligence cooperation with the U.S. and other NATO forces, according to the statement issued after the defense committee meeting.
The White House said that senior U.S. civilian and military officials had expressed their condolences to their Pakistani counterparts.
The White House statement said the officials expressed "our desire to work together to determine what took place, and our commitment to the U.S.-Pakistan partnership which advances our shared interests, including fighting terrorism in the region."
The White House statement did not address Pakistan's decision to block supply routes for the war in Afghanistan or its demand that the U.S. vacate the drone base.
The Pakistani army said Saturday that NATO helicopters and fighter jets carried out an "unprovoked" attack on two of its border posts in the Mohmand tribal area before dawn, killing 24 soldiers and wounding 13 others. The troops responded in self-defense "with all available weapons," an army statement said.
Pakistan army chief Gen. Ashfaq Parvez Kayani condemned the attack, calling it a "blatant and unacceptable act," according to the statement.
A spokesman for NATO forces, Brig. Gen. Carsten Jacobson, said Afghan and coalition troops were operating in the border area of eastern Afghanistan when "a tactical situation" prompted them to call in close air support. It is "highly likely" that the airstrikes caused Pakistani casualties, he told BBC television.
"My most sincere and personal heartfelt condolences go out to the families and loved ones of any members of Pakistan security forces who may have been killed or injured," Gen. John Allen, the top overall commander of U.S. and NATO forces in Afghanistan, said in a statement.
The border issue is a major source of tension between Islamabad and Washington, which is committed to withdrawing its combat troops from Afghanistan by the end of 2014.
Much of the violence in Afghanistan is carried out by insurgents who are based just across the border in Pakistan. Coalition forces are not allowed to cross the frontier to attack the militants. However, the militants sometimes fire artillery and rockets across the line, reportedly from locations close to Pakistani army posts.
American officials have repeatedly accused Pakistani forces of supporting ? or turning a blind eye ? to militants using its territory for cross-border attacks. But militants based in Afghanistan have also been attacking Pakistan recently, prompting complaints from Islamabad.
The two posts that were attacked Saturday were located about 1,000 feet (300 meters) apart on a mountain top and were set up recently to stop Pakistani Taliban militants holed up in Afghanistan from crossing the border and staging attacks, said local government and security officials.
There was no militant activity in the area when the alleged NATO attack occurred, local officials said. Some of the soldiers were standing guard, while others were asleep, they said, speaking on condition of anonymity because they were not authorized to talk to the media.
Pakistan army spokesman Maj. Gen. Athar Abbas said map references of all of the force's border posts have been given to NATO several times.
Pakistan's prime minister summoned U.S. Ambassador Cameron Munter to protest the alleged NATO strike, according to a Foreign Ministry statement. It said the attack was a "grave infringement of Pakistan's sovereignty" and could have serious repercussions on Pakistan's cooperation with NATO.
Munter said in a statement that he regretted any Pakistani deaths and promised to work closely with Islamabad to investigate the incident.
The U.S., Pakistan, and Afghan militaries have long wrestled with the technical difficulties of patrolling a border that in many places is disputed or poorly marked. Saturday's incident took place a day after a meeting between NATO's Gen. Allen and Pakistan army chief Gen. Ashfaq Parvez Kayani in Islamabad to discuss border operations.
The meeting tackled "coordination, communication and procedures ... aimed at enhancing border control on both sides," according to a statement from the Pakistani side.
The U.S. helicopter attack that killed two Pakistani soldiers on Sept. 30 of last year took place south of Mohmand in the Kurram tribal area. A joint U.S.-Pakistan investigation found that Pakistani soldiers fired at the two U.S. helicopters prior to the attack, a move the investigation team said was likely meant to notify the aircraft of their presence after they passed into Pakistani airspace several times.
A U.S. airstrike in June 2008 reportedly killed 11 Pakistani paramilitary troops during a clash between militants and coalition forces in the tribal region.
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Associated Press writers Anwarullah Khan in Khar, Pakistan, Riaz Khan in Peshawar, Pakistan, Matiullah Achakzai in Chaman and Deb Riechmann in Kabul, Afghanistan, contributed to this report.
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